|
|
|
|
| |
|
|
| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
| |
|
|
| Torre,D De la; Nuñez,LFN; Puga,B; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. |
| ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken parvovirus; Enteric diseases; Molecular diagnostic; PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400643 |
| |
|
|
|
